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您所在的位置:北京索莱宝科技有限公司网站500万彩票>>产品展示>>细胞相关产品>>动物血清>>四季青 无噬菌体,低内毒素特级胎牛血清
 
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四季青 无噬菌体,低内毒素特级胎牛血清

产品型号:
产品报价:
产品特点:
四季青 无噬菌体,低内毒素特级胎牛血清
规格:100ml
品牌:四季青
血清通常作为补充成分添加在基础培养基内使用。它能够提供各种大分子蛋白,小分子量营养,水溶性成分的转运蛋白,和其它一些细胞在体外所必需的成分,如激素和附着因子。血清可以结合或中和一些生长因子中的有毒成分,并提供培养基的缓冲能力。细胞培养时血清的添加依赖于基础培养基的化学组成,培养细胞的类型,及所用的培养系统。
  四季青 无噬菌体,低内毒素特级胎牛血清的详细资料:

无噬菌体,低内毒素特级胎牛血清 

规格:100ml
品牌:四季青

四季青 无噬菌体,低内毒素特级胎牛血清通常作为补充成分添加在基础培养基内使用。它能够提供各种大分子蛋白,小分子量营养,水溶性成分的转运蛋白,和其它一些细胞在体外所必需的成分,如激素和附着因子。血清可以结合或中和一些生长因子中的有毒成分,并提供培养基的缓冲能力。细胞培养时血清的添加依赖于基础培养基的化学组成,培养细胞的类型,及所用的培养系统。


WOMENZAITIGONGXIBAOPEIYANGJI、PINGHENGYANRONGYE、WUXUEQINGPEIYANGJIHESHIJIDETONGSHI,HAITIGONGGAOPINZHIDEXUEQING。WOMENDUIXUEQINGZHIBEIJINXINGQUANGUOCHENGJIANGUAN。CONGXUEQINGSHOUJIDAOZHONGCHANPINJIANYANHENEIBUJIHEDEMEIYIGEBUZHOU,WOMENDOUCAIYONGZHUANYONGSHEBEIHEBIAOZHUNCAOZUOCHENGXU,YIQUEBAOZHENGGEGUOCHENGZHONGMEIGEHUANJIEDECHANPINZHILIANG。DUIZHENGGEGUOCHENGDEKONGZHIKEYIBAOZHENGCHANPINDECHIXUGAOPINZHI,JIANGDIBUTONGPICIDECHAYI。


CHULI:QUANBUXUEQING:SHOUJIDEXUEYEJUNZAILENGZANGTIAOJIANXIACHULI。XUEQINGHEXUEKUAIFENLIHOULIJIJIANGXUEQINGHEBINGBINGLENGDONG。ZHIYOUFUHEWOMENDEJIANCEBIAOZHUNDEXUEQINGCAIBEIJIESHOUZUOJINYIBUCHULI。


REMIEHUOXUEQING:REMIEHUOSHIZAI56℃SHUIYUZHONGYANGEKONGWEN30FENZHONG。TOUXIXUEQING:YONG10,000JIELIUFENZILIANGDETOUXIMOZAI0.15MDENaClZHONGJINXINGTOUXI,ZHIDAOPUTAOTANGSHUIPINGDIYU5mg/dl(YONGLINJIABENANCESHIFAJIANYAN)。γSHEXIANZHAOSHEXUEQING:KEANKEHUYAOQIUDUIXUEQINGJINXINGγSHEXIANZHAOSHE。TONGGUOγSHEXIANZHAOSHEYIMIEHUOGEZHONGDONGWUXUEQING(BAOKUOTAINIUXUEQING、XINSHENGNIUXUEQING、ZHUXUEQINGHEMAXUEQING)ZHONGDEBINGDUHEZHIYUANTIDEFANGFAYIJINGDEDAOZHENGSHI。YANJIUZHENGSHIZHAOSHEJILIANGZAI30-45kGyWEIYI。XUEQINGZAICIZHAOSHEHOUWULIHUAXUETEXINGHEXIBAOPEIYANGBIAOXIANMEIYOUBIANHUA。ZHUANGPING:CUXUEQINGXUTONGGUOYIXILIEDEGUOLVCAICHENGWEICHENGPIN。HOUDEGUOLVBUZHOUBAOKUOSHIYONG0.2umHE0.1umDEWUJUNGUOLV。TAINIUXUEQINGXUTONGGUOSANCI0.1umGUOLV,QITAXUEQINGXUTONGGUO0.2umGUOLV。ZHONGCHANPINDEZHILIANGKONGZHI:WOMENCAIQUYIXIAFANGFADUIMEIYIPICIDECHANPINXUANQUYIDINGSHULIANGDEYANGPINJINXINGZHILIANGKONGZHIFENXI:


HUAXUEJIANCE:SHIYONGDIWENNINGGUDIANDEFANGFAJIANCESHENTOUYA,YIBAOZHENGFUHECHANPINGUIFAN。WOMENMEITIANSHIYONGGUOJIABIAOZHUNDEJISHUYANJIUSUOBIAODINGDEBIAOZHUNRONGYEDUIWOMENDESHENTOUYAJIANCEYIQIJINXINGXIAOZHUN。TONGYANGMEITIANSHIYONGGUOJIABIAOZHUNJISHUYANJIUSUOBIAODINGDEBIAOZHUNRONGYEDUIpHJIJINXINGXIAOZHUN。


SHIYONGDIANYONGTUPUJIANDINGXUEQINGDEZHENGTIXINGZHI。YANGPINBEIZHUANYIDAOXIAOSUANXIANWEISUJIZHIZHONG,BINGZAIHUANCHONGTIXINEIQIANYI。TIAODAIJINGRANSE、QINGJIE、GANZAOHOUYONGMIDUYIJINXINGSAOMIAO,YIJIANCEBAIDANBAIHEQIUDANBAIZUFENDEXIANGDUINONGDU。WEIZHENGSHISHIFOUZAISHIDANGDETIAOJIANXIAJINXINGCAIXUEHECHULI,SHIYONGXIUSHIDEFlemingFANGFADUIXUEHONGDANBAIJINXINGFENGUANGGUANGDUJIANCE。


SUIZHEDONGWUNIANLINGDEZENGJIA,XUEQINGZONGDANBAIHANLIANGYEXIANGYINGDITIGAO。SHIYONGZIDONGHUADEBiuretFANGFAJINXINGZONGXUEQINGDANBAIDEJIANCE,YIQUERENDONGWUNIANLINGBINGYANZHENGFUHECHANPINGUIFAN。SHIYONGSEDUJIZAIBUTONGBOZHANGXIAJIANCEYANGPINHEBiuretSHIJIHUNHEBODEXIGUANGDU。ZIDONGJISUANJIEGUOHOU,YUBIAOZHUNQUXIANBIJIAO,JIKEDEDAODANBAIZHI(KE/GONGSHENG)。


WENDINGXINGJIANCECHENGXU:ZAIMEIYIGEBIAOJIWEITIWAIZHENDUANDECHANPINSHANGXIANSHIDEXIAOQIBIAOMINGLEZHEIGECHANPINJUYOUDUOZHANGSHIJIANDEXIAONENG。WOMENDEWENDINGXINGCHENGXUQUEDINGHEZHENGMINGLECHANPINXIAOQI。WOMENDINGQIJIANCEPILIANGCHANPIN,QUEDINGCHANPINZAITUIJIANZHUCUNTIAOJIANXIAJUYOUKEJIESHOUXIAOGUODESHIJIANZHANGDU。


500万彩票WEISHENGWUXUEJIANCE:SUOYOUXUEQINGSHIYONGBarile&KernDEDATIJIFANGFAJIANCEZHIYUANTI。ZAITUIJIANFANGFADEJIANCEFANWEINEIJIANCEJIEGUOSHIZHUNQUEDE。YANGPINZHISHAOYINGGAIZAIROUTANGZHONGHEBINGPEIYANG3GEXINGQI,TONGSHIYAOZAIQIONGZHIPINGBANSHANGJINXINGBUSHAOYU4CICHUANDAIPEIYANG。ZAIYOUYANGHEYANYANG(95%DAN,5%CO2)TIAOJIANXIA,PINGBANZAI36±2℃XIAFUYU。ZAIJIANCEGUOCHENGZHONG,MEIZHOUDUIQIONGZHIPINGBANJINXINGYICIWEISHENGWUSHENGZHANGQINGKUANGJIANYAN。SHIYONGDienesRANSEFADUIHUAIYIBEIWURANDEQIONGZHIPINGBANJINXINGZHIYUANTIJUNLUODEJIANCE。RANHOU,DUIPINGBANJINXINGZAICIJINGJIAN。DUIFUYUROUTANGPINGYAODINGQIJINXINGZHUODUHEpHZHIBIANDONGDEJIANCE。ZAITUIJIANFANGFAJIANCEFANWEINEI,SUOYOUXUEQINGYEYAOSHIYONGHoechstYINGGUANGDNARANSEFAJINXINGBUKEZAIROUTANGZHONGPEIYANGDEZHIYUANTI(BAOKUOM.hyorhinisSHU)JIANCE。


500万彩票BINGDUJIANCE:YIZHIWUWAILAIWUZHIDEXIBAOPEIYANGJIXUJINGGUOZAIBAOHAN15%CESHIXUEQINGDESHENGZHANGPEIYANGJIZHONGJINXINGWEIQI21TIANDESANCICHUANDAIPEIYANG。SHIYONGDUIZHAOPEIYANGJIHEYIZHIDUIWAILAIWUZHIWEIYINXINGDEXUEQINGPINGXINGDIWEICHIYINXINGDUIZHAOPEIYANG。ZHENGGEQIJIAN,JIANCESUOYOUDEPEIYANGQINGKUANG,YIBIANFAXIANSHIFOUCUNZAIBINGDUYOUFADEXIBAOXINGTAIGAIBIANHUOZHIXIBAOBINGBIANXIAOYING。ZAI14ZHI21TIANDEPEIYANGQIJIAN,DUIHANYOUJIANCEXUEQINGDEPINGXINGPEIYANGPINGANXIAMIANBIAOZHUNBINGDUJIANCEFANGFAJINXINGPINGGU。


500万彩票JIANGQIZHONGYIPINGJIANCEXIBAOYONGYIDANBAIMEIXIAOHUA,RANHOUBAXIBAOFENBIEJIARUDAOZONGMIANJIWEI6cm 2 DELIUSHIZAIBOPIANSHANG。TONGSHIZHUNBEIYINXINGDUIZHAOZAIBOPIAN。ZAIJIANCEPEIYANGDEDANSHIZAIBOPIANSHANGJIARUNIUBINGDUXINGFUXIEBINGDU(BVDV)、NIUXIXIAOBINGDU、NIUXIANBINGDU、KUANGQUANBINGDUHEHUCHANGBINGDUDERANSEJI,ZUOWEIDANGEDEYANGXINGDUIZHAO。ZAIJINGGUO7TIANPEIYANG(ZONGGONG21TIAN),LIYONGYINGGUANGKANGTIJISHUJIANCEBINGDU。


TONGGUODUIJIANCEPEIYANGJINXINGSUMUJINGYIHONGRANSE,GUANCHAZHIXIBAOTUBIANXIAOYING(CPE)HUOQITABINGDUYOUDAOXIAOYING。JIANCEXIBAOSHIFOUCUNZAICPEXIAOYING、NEIHANWU、DUOHETIHUOQITAYICHANGXIAOYING。


500万彩票NEIDUSUJIANCE:WOMENYONGAMIBABIANXINGXIBAORONGJIEWU(LAL)NINGJIAOFALAIJIANCETAINIUXUEQINGDENEIDUSUHANLIANG。


效能检测:
500万彩票对每一批次的胎牛血清,我们都进行下述培养效能检测。选择血清重要的标准是其支持特殊细胞的生长能力。因此,除了保证血清通过严格的品质控制,我们也同时在实验室条件下对血清的三个重要的培养效能进行检测:克隆效率、贴壁效果和二倍体成纤维细胞生长促进。


克隆效率分析:克隆效率实验分析每一批胎牛血清促进小鼠骨髓瘤细胞及其衍生的杂交瘤细胞的克隆和生长能力。下列产品
经验证可用于该实验:
Sp2/0-Ag14(ATCC #CRL 1581)
P3x63-Ag8.653(ATCC #CRL 1580)


MEIYIPITAINIUXUEQINGDOUYAOFENBIEZAIFUZHIWEILIANGDIDINGPINGBANSHANGJIARULIANGZHONGXUEQINGNONGDUHELIANGZHONGXIBAOJIEZHONGLIANG(10%XUEQINGHE1cell/KONG,4%XUEQINGHE5cells/KONG)DEQINGKUANGXIAJINXINGFENXI。KELONGFENXIJIEGUOCHUYIYIQUEDINGDECANZHAOXUEQINGZHIDEYIGUIYIHUA。ZAIXUDUORICHANGZAJIAOXUANZECHENGXUZHONGJUYOUDAIBIAOXINGDESHIGAONONGDUDEXUEQING,ERDINONGDUXUEQINGZAISHEJIXUEQINGPICIXIWEICHAYIFANGDAJIANCEZHONGYOUGENGWEIYANGEDECESHI。YANGEDE1cell/KONGCESHISHIMONIDANYIZAJIAOXUANZEDESHIYANSHITIAOJIAN。WEIMEIYIPICITAINIUXUEQINGTIGONGDEFENXIZHENGMINGBAOGAOZHONGDEKELONGXIAOLVZHIWEILIANGCICESHITIAOJIANXIADEPINGJUNZHI。


克隆分析法:克隆分析法是在复式96孔板中加入两种胎牛血清浓度(10%v/v)和(4%v/v)和两种接种量的情况下进行的。每一次化验中,同时测量前期已验证合格的一批胎牛血清,并将此测量值作为内部参考标准。
克隆分析按下述方法进行:


500万彩票1.Sp2/0-Ag14(sp2)HEP3x63-Ag8.653(653)ZAIHANYOU10%DETAINIUXUEQINGDERPM1640ZHONGTONGCHANGBAOCHIDUISHUSHENGZHANGQI。ZAIXIANWEIJINGXIA,LIYONGTAIPANLANPAICHUFADUIHUOXIBAOJINXINGJISHU。


2.YONGRPM1640PEIYANGJIJIANGCHUBEIXIBAOXUANFUYEJINXINGLIANXUXISHI,SHIQIDADAOYUQIDE25cells/mlHE5cells/mlJIEZHONGNONGDU。ZHUNBEIHANYOUCANZHAOHUOCESHITAINIUXUEQINGDERPM1640PEIYANGJI。JIANGZHEIZHONGMIDUDEXIBAOFENPEIDAOGEGEFENXISHENGZHANGPEIYANGJITIJIWEI0.2HAOSHENGDEKONGZHONG,SHIQIFENBIEPINGJUNHANYOU5GEHE1GEXIBAO。


3.JIANG96KONGBANFANGRU36±2℃,4-6% CO 2 DECHAOSHIPEIYANGXIANGFUYUYUE10ZHI15TIAN。


4.ZAIFUYUQIJIAN,YONGROUYANGUANCHAPINGBANBINGDUIYONGYUKELONGDEKONGJINXINGJISHU。KELONGXIAOLVANXIALIEFANGFAJISUAN:KELONGXIAOLV=(YANGXINGKONGPINGJUNSHU/FUYUKONGZONGSHU)×100%


500万彩票5.MEIYIPICITAINIUXUEQINGWEICHIZHISHIJIGUSUILIUXIBAOKELONGXIAOLVDEDINGLIANGANZHAOYIXIAFANGFACEDEDEXIANGDUIKELONGXIAOLV(RCE)JINXINGGUIYIHUA:RCE=JIANCEXUEQINGDEKELONGXIAOLV/DUIZHAOXUEQINGDEKELONGXIAOLVTIEBIXIAOLVFENXI:TIEBIXIAOLVFENXISHILIYONGYIZHUANXINGDERENLEIXIBAOXILAIJIANCEMEIYIPIXUEQINGCUSHICHIXUTIEFUXIBAOXIDESHENGZHANGNENGLI。XUANZEYIBEIYANZHENGKEYIJIANCETIEBIXIAOLVDEXIBAOXIA549(RENFEIZHONGLIUXIBAO,ATCC#CCL,185),SHIYINWEITAKEYIQUFENYIPIXUEQINGWEICHIXIBAOTIEBIHEFANZHINENGLIDEXIWEICHAYI。


MEIYIPICITAINIUXUEQINGDOUYAOZAILIANGZHONGXUEQINGNONGDUHELIANGZHONGXIBAOJIEZHONGNONGDUXIA(10%XUEQINGHE100cells/KONG,4%XUEQINGHE200cells/KONG)CESHISANCI。ZAI36±2℃,4-6% CO 2 DECHAOSHIPEIYANGXIANGFUYUYUE10ZHI14TIAN,DUIBEIRANSEDEXIBAOJUNLUOJINXINGJISHUJIKEDEDAOQITIEBIXIAOLV。JIANGJIEGUOCHUYICANKAODEDUIZHAOXUEQINGDEYIGUIYIHUA。TONGGUOLIANGZHONGXUEQINGNONGDUDECESHI,KEYIYANZHENGGAIPICIXUEQINGZAIJIANLIANGHEBIAOZHUNSHUIPINGXIAWEICHISHENGZHANGDENENGLI。WEIMEIYIPICITAINIUXUEQINGTIGONGDEFENXIZHENGMINGBAOGAOZHONGDETIEBIXIAOLVZHIWEILIANGCICESHITIAOJIANXIADEPINGJUNZHI。


TIEBIFENXIFA:SHIYONGTIEFUFENXIJIANCESHANGSHUMEIYIPICESHIXUEQING。ZHEIZHONGFENXIFAZHENDUIMEIYIGEXUEQINGYANGPINSHIYONGYIGE6KONGBAN(MEISHI9.6cm 2 ),BINGKEYIDUISANGEKONGDESHUJUJINXINGPINGJUNCHULI。MEIYICIHUAYANZHONG,TONGSHICELIANGQIANQIYIYANZHENGHEGEDEYIPITAINIUXUEQING,BINGJIANGCICELIANGZHIZUOWEINEIBUCANKAOBIAOZHUN。


TIEFUFENXIANXIASHUFANGFAJINXING:


500万彩票1.A549XIBAOXIYONGHANYOU10%TAINIUXUEQINGDEDMEMPEIYANGJI,ZAI36±2℃,YIYARONGHEMIDUXIAJINXINGPEIYANG。


500万彩票2.HAN10%(v/v)HE4%(v/v)XUEQINGDEDMEMYOUFENBIEZAI22.5HAOSHENGHE24HAOSHENGDMEMZHONGJIARU2.5HAOSHENGHE1HAOSHENGTAINIUXUEQINGPEIZHIERCHENG,ZHONGYOUXIAOTIJIWEI25HAOSHENG。JIANG5HAOSHENGHANXUEQINGPEIYANGJIFENBIEJIARU6KONGBANDEMEIYIGEKONGZHONG。


3.A549PEIYANGXIBAOXIANJINGGUOYIDANBAIMEIXIAOHUA,CEDINGHUOXIBAOSHU,RANHOUXISHIXIBAOXUANFUYEZHI2,000cells/ml。


500万彩票4.JIANG0.1HAOSHENGXIBAOXUANFUYEJIARU200cells/KONGSHIZHONG,0.05HAOSHENGXUANFUYEJIARU100cells/KONGSHIZHONG。PINGBANHUNHEHOUFANGRU36±2℃,5%CO 2 DECHAOSHIPEIYANGXIANGFUYUYUE10ZHI14TIAN。


500万彩票5.FUYUHOU,QUCHUPINGBAN,QUCHUSHENGZHANGPEIYANGJI,YONGYAJIAJILAN-JIACHUNRONGYEDUIJUNLUOJINXINGRANSE。


500万彩票6.JISUANJUNLUOXINGCHENG,RANHOUANXIASHUFANGFAJISUANTIEBIXIAOLV:%TIEBIXIAOLV=MEIKONGJUNLUOPINGJUNSHU/MEIKONGFUYUHUOXIBAOPINGJUNSHU×100


7.WEIFANGBIANBIJIAO,JIANGCEDEDETIEBIXIAOLVSHUZHICHUYIMEIPIJIANCEXUEQINGDEXIANGDUITIEBIXIAOLV(RPE)DEYIGUIYIHUA:RCE=JIANCEXUEQINGDETIEBIXIAOLV/CANZHAOXUEQINGDETIEBIXIAOLVERBEITICHENGXIANWEIXIBAOSHENGZHANG:CUJINSHENGZHANGFENXIYONGYUJIANCEMEIYIPITAINIUXUEQINGWEICHINANPEIYANGDERENERBEITICHENGXIANWEIXIBAOZHANGQISHENGZHANGDENENGLI。


500万彩票XIALIEXIBAOXIJINGRENZHENGKEYONGYUGAISHIYAN:


WI-38(RENERBEITIFEICHENGXIANWEIXIBAO,ATCC#CCL 75)


500万彩票WI-38XIBAOZAIHAN5%TAINIUXUEQINGDEDIMIDU(2X10 5 /PING)FUSHI25-cm 2PINGZHONGFUYU,BINGJINXINGWEIQISANGELIANXU7TIANDECHUANDAIPEIYANG。ZAIMEIYIGEPEIYANGQI,XIBAODOUCONGCHUSHIFUYUMIDUKAISHICHUANDAIPEIYANG。YALIFENCENGLV、CHENGBEIFANZHISHULIANGHEJIANLIANGXUEQINGNONGDUSHIMEIPICICUJINNANPEIYANGERBEITIXIBAODESHENGZHANGXUEQINGDEYANGECESHIZHIBIAO。TONGGUOLIANXUSANDAIPEIYANGYIJIANSHAOQIANYICISHENGZHANGPEIYANGJIDECANLIUYINGXIANG,CONGERBAOZHENGDEDAOJIANCEPICUJINSHENGZHANGNENGLIDEZHENSHIJIEGUO。WEIMEIYIPICITAINIUXUEQINGTIGONGDEFENXIZHENGMINGBAOGAOWEIDISANCICHUANDAIPEIYANGMEIFUSHIPEIYANGPINGXIBAOSHUDEPINGJUNZHI。JIANGJIANCEZHICHUYICANKAODUIZHAOZHIDEYIGUIYIHUA。


500万彩票ERBEITICHENGXIANWEIXIBAOSHENGZHANGFENXIFA:PEIYANGSHUDAIWI-38RENLEIERBEITIFEICHENGXIANWEIXIBAO,JISUANMEIYIDAIDEXIBAOZONGSHU。CIXIANGJIANCESUOTIAOXUANCHUDETAINIUXUEQINGPIHAONENGWEICHINANYISHENGZHANGXIBAO、TIEBIXINGXIBAO、ZHENGCHANGDEXIBAOZHUSHENGZHANGJICUNHUO。


500万彩票1.ZHUNBEIHAN5%JIANCEXUEQINGHUOYIYANZHENGHEGEDECANZHAOXUEQINGSHENGZHANGPEIYANGJI,JICHUSHENGZHANGPEIYANGJIWEIHANL-GUANXIANANDEHBME:EBME(1:1)。ANYAOQIU,ZAIMEIYICILIULIANGBIANHUAHEFENXIGUOCHENGZHONGJINXINGCHUANDAIPEIYANGSHIXUPEIZHIHAN5%XUEQINGSHENGZHANGDEPEIYANGJI。


2.ZAIDIDAIPEIYANGSHUIPINGSHI,JIANG2X10 5 WI-38XIBAOJIARUGEGEHAN9.5HAOSHENGSHENGZHANGPEIYANGJIDEFUSHI25-cm 2 PEIYANGJIZHONG。ZAIBUNINGJINPINGGAIDEQINGKUANGXIA,JIANGPEIYANGPINGFANGRU4-6%CO 2, 36℃±2℃HUANJINGZHONG,BAOCHI24XIAOSHI。RANHOUNINGJINPINGGAI,JIANGPEIYANGPINGFANGZAI36℃±2℃XIAFUYU7TIAN,ZAIDI2TIANHUODI3TIANQUANBUGENGHUANPEIYANGJI。


3.ZAIDI7TIAN,YONGYIDANBAIMEIDUIMEIYIGEHANCANZHAOHUOJIANCEXUEQINGDEFUSHIPINGZHONGDEXIBAOJINXINGXIAOHUADEDAOYIDINGSHULIANGXIBAO,RANHOUHEBINGBINGJISHU。JIANG2x10 5 WI-38XIBAOJIARUHANYOUXINXIANSHENGZHANGPEIYANGJI(YIJIARULEYUSHANGYIDAITONGYIPICIDECANZHAOHUOJIANCETAINIUXUEQING)DEXINDEFUSHI25-cm 2 PINGZHONG,JINXINGXIBAOCHUANDAIPEIYANG。ANDI2BUSUOSHU,JIANGPEIYANGPINGJINXINGPINGHENGBINGFUYU。


500万彩票4.RUSHANGSUOSHUJINGGUOSANDAILIANXUFENXI,ZAIMEIYIDAIJIESHUSHIJISUANMEIYIPICANZHAOHUOJIANCEXUEQINGDEMEIPINGXIBAOPINGJUNSHU。HOUYIDAIPEIYANGDEGEPIJIANCEXUEQINGSHIYANZHONGDEXIANGDUISHENGZHANGLV(RGR)ZUOWEICANZHAOXUEQINGSHIYANZHONGHUODEDEXIBAOSHENGZHANGLVFENZI:


500万彩票%RGR=JIANCEXUEQINGSHIYANZHONGMEIPINGPINGJUNXIBAOSHU/CANZHAOXUEQINGSHIYANZHONGMEIPINGPINGJUNXIBAOSHUX100S f9XIBAOSHENGZHANGCUJINFENXI。TONGGUOKUNCHONGFENXIKEYIBAOZHENGNINGOUMAIDETAINIUXUEQINGPICIKEYIWEICHIS f9XIBAODESHENGZHANG。SHOUDAOCHANPINHOU,NINXUYAOZUODEJIUSHIHUNYUNXUEQING,ZAI56℃XIAREMIEHUO30FENZHONG。ZHEIZHONGFENXIFAYEKEYIYONGYURUBAIDANBAISHUIJIECHANPIN、JIAOMUHEQITASHENGZHANGFULIAOZUOWEIYUANLIAODESHENGWUXIAONENGFENXI。GAIFENXIFADEZHUNQUEXINGQUJUEYUSHIFOUYONGCHANGGUIDEXIBAOPEIYANGFANGANJINXINGPEIYANG。YONGTAIPANLANRANSEPAICHUFACEDEDEXIBAOHUOXINGZHISHAOBUDIYU80%。


XIBAOSHENGZHANGFENXIFA。


500万彩票1.SHIYONGHAN10%JIANCEHUOCANZHAOREMIEHUOTAINIUXUEQINGDEYIYANZHENGDEGraceKUNMINGPEIYANGJIPEIZHIWANQUANJIANCEPEIYANGJI。


2.JIANGCHUBEISf9XIBAOJIARU50HAOSHENGLIXINGUANZHONG,ZAI50XgXIALIXIN5FENZHONG。RANHOUJIANGMEIZHISHIGUANDECHENDIANWUZHONGXINYONGHAN10%DEJIANCEHUOCANZHAOREMIEHUOTAINIUXUEQINGWANQUANPEIYANGJIPEIZHICHENGXUANFUYE。


3.FANSHIGUANDIANDAOJICI,RANHOUMEIZHISHIGUANDAOCHU1HAOSHENGYANGPIN。YONGTAIPANLANRANSEPAICHUJINXINGHUOXIBAOJISHU。


4.RUGUOHUOXING≥80%,BASHIGUANZAIDIANDAOJICIBINGQUCHU0.25HAOSHENGYANGPIN。RANHOUYONGCoulterJISHUQIJINXINGXIBAOJISHU。


500万彩票5.JIANGXIBAOJIARUErlenmeyerPINGZHONG,SHIQIZHONGXIBAONONGDUWEI2.75X10 5 cells/ml。FUYUHOU,ZAIYICIJINXINGXIBAOJISHU,YIBAOZHENGXIBAOMIDUWEI2.5X10 5 ZHI3.0X10 5 cells/ml。


6.JIANGPEIYANGPINGFANGDAOYAOCHUANGSHANG,ZAI27℃±1℃、80-90rpmTIAOJIANXIAFUYU96XIAOSHI。


500万彩票7.CONGMEIYIPEIYANGPINGZHONGFENBIEQUCHU0.25HAOSHENGYANGPIN,YONGCoulterJISHUQIJINXINGJISHU。TONGSHIQUCHUYIDINGLIANGDEYANGPINJINXINGXIBAOHUOXINGJIANCE。


8.JIANGJIANCEXIBAOMIDUYUCANZHAOXIBAOMIDUXIANGBIJIKEDEDAOXIANGDUIXIBAOMIDU(RCN)。


500万彩票SHIJUNTIJIANCE:WOMENLIYONGRONGJUNBANFENXIFALAIJIANCESHIFOUCUNZAISHIJUNTI。CONGMEIYIPIXUEQINGZHONGQUJUDAIBIAOXINGDEYANGPINJIARUYIDANBAIMEILINSUANQIONGZHIZHONG,BINGZAIQIJIARUHANYOUSHIJUNTIMINGANDEDACHANGGANJUNXUANFUYE(C-3000HUOK-12)。RANHOU,HUNHEWUHUIZAIYIDANBAIMEILINSUANQIONGZHIPINGBANSHANGFENCENG,ZAI36±2℃HUANJINGXIAFUYUYUE24XIAOSHIHOU,GUANCHAPINGBANSHANGRONGJUNBANDEXINGTAI。


SHENGHUATEZHENGJIANCE。

碱性磷酸酸酶 葡萄糖
重碳酸盐高密度脂蛋白(HDL)
胆红素(总)乳酸脱氢酶(LDH)
血脲氮(BUN)低密度脂蛋白(LDL)
BUN/肌氨酸酐比钾磷(无机)
血清谷草转氨酶
氯化物
胆固醇总铁结合力
肌氨酸酐转铁蛋白
γ-谷氨酰基转移酶甘油三酸酯(TG)
尿酸


四季青 无噬菌体,低内毒素特级胎牛血清检测:所有批次的胎牛血清我们都进行了血红蛋白的检测,血红蛋白含量低于《中国生物制品主要原辅材料指控指标》(2000年版)公布的指标。


XIAONENGJIANCE:QITAXUEQING


500万彩票XINSHENGNIUXUEQING。JIANCEXINSHENGNIUXUEQINGWEICHICHAOGUOSANDAIVEROXIBAOSHENGZHANGDENENGLI。ZAIMEIYIGEPEIYANGQI,XIBAODOUCONGCHUSHIFUYUMIDUKAISHICHUANDAIPEIYANG。KEJIANGSUODEJIEGUOYUSHIYONGHANYOUYIZHITEZHENGDECANZHAOXUEQINGDUIZHAOSHENGZHANGPEIYANGJIHUODEDEPINGXINGJIEGUOJINXINGBIJIAO。


MAXUEQING。MEIYIPIMAXUEQINGBEIJIANCEWEICHIZAIHANYOUJIANCEPIXUEQINGDEDUIZHAOPEIYANGJISHANGSp2/0-Ag14(XIAOSHUGUSUILIU)XIBAOSHENGZHANGDENENGLI。KEJIANGSUODEJIEGUOYUSHIYONGHANYOUYIZHITEZHENGDECANZHAOXUEQINGDUIZHAOSHENGZHANGPEIYANGJIHUODEDEPINGXINGJIEGUOJINXINGBIJIAO。


XUEQINGDESHIYONGYUCHUCUN:ZHENGQUEDESHIYONGJIBAOCUNXUEQING,CAINENGSHIXUEQINGFAHUIYINGYOUDEZUOYONG。


1. CHUCUNTIAOJIAN:XUEQINGYIBANCHUCUNYU-20℃,TONGSHIYINGBIMIANFANFUDONGRONG。GOUMAIDABAOZHUANGDEXUEQINGHOU,SHOUXIANYAOMIEHUOCHULI,RANHOUFENZHUANGCHENGXIAOBAOZHUANG,CHUCUNYU-20℃,SHIYONGQIANRONGHUA。RONGHUASHIHAOXIANZHIYU4℃。RONGHUAHOUDEXUEQINGZAI4℃BUYIZHANGSHIJIANCUNFANG,YINGJINKUAISHIYONG。


500万彩票2. SHIYONGNONGDU:ZICONGYOULEHECHENGPEIYANGJI,XUEQINGJIUSHIZUOWEIYIZHONGTIANJIACHENGFENYUHECHENGPEIYANGJIHUNHESHIYONG,SHIYONGNONGDUYIBANWEI5-20%,CHANGYONGSHI10%。GUODUOXUEQINGRONGYISHIPEIYANGZHONGDEXIBAOFASHENGBIANHUA,TEBIESHIYIXIEERBEITIDEWUXIANXIBAOXI,XUNSUSHENGZHANGZHIHOURONGYIFASHENGEXINGZHUANHUA。


关于使用中的常见问题


1.保存血清好的办法?
500万彩票我们建议血清应保存在-5℃至-20℃。若你一次无法用完一瓶,建议您无菌分装血清至恰当的灭菌容器内,再放回冷冻。


2.如何解冻血清才不会使产品质量受损?
我们建议您将血清从冷冻箱中取出后,先置于2-8℃冰箱中使之溶解,然后在室温下使之全溶。但必须注意的是,溶解过程中必须规则地摇晃均匀。


3.血清解冻后发现有絮状沉淀物出现,该如何处理?
血清中沉淀物的出现有许多原因,但普遍的原因是由于血清中脂蛋白的变性造成的;而血纤维蛋白(形成凝血的蛋白之一)在血清解冻后,也会存在于血清中,亦是造成血清沉淀物的主要原因之一。但这些絮状沉淀物,并不影响血清本身的质量。


500万彩票若您欲去除这些絮状沉淀物,可以将血清分装至无菌离心管中,以400g稍微离心,上清液即可接着加入培养基内一起过滤。我们不建议您以过滤的方法去除这些絮状沉淀物,因为它可能会阻塞您的过滤膜。


4.何谓热灭活?有必要做热灭活吗?
一般以56℃,30分钟水浴来处理已解冻的血清,因为此加热步骤,可以使补体去活性,而补体所参与的反应有:溶细胞活性,平滑肌的收缩,肥大细胞和血小板组胺的释放,增强吞噬作用,淋巴细胞、巨噬细胞的趋化和激活。实验显示,经过正确处理的热灭活血清,对大多数的细胞而言是不需要的。经过处理的血清对细胞生长只有微小的促进,或完全没有任何作用,甚至通常因为高温处理影响了血清的质量,而造成细胞生长速率的降低。


而经过热处理的血清,沉淀物的形成会显著的增多,这些沉淀物在倒置显微镜下观察,像是“小黑点",常常会让研究者误以为是血清遭受污染,而把血清放在37℃环境中,又会使此沉淀物更增多,使研究者认为是微生物的分裂扩增。因此我们建议您,若非必须,您可以不需要做热灭活这一步。如此以来,不但节省您宝贵的时间,更确保您血清的质量!


5.如何避免沉淀物的出现?
我们建议您在使用血清的时候,注意正确的血清解冻步骤,并尽量避免灭活血清及长时间的将血清置于高温环境中。


产品订购说明:
1、绝大部分产品备有现货,一般情况下都能订货当日发货。 
2、部分非常用产品,需提前1-2日预订,海外期货则需要提前3-6周预订。 
3、部分产品价格会因货期、批次等因素发生变化,若有变动以订货当日新价格为准。 
4、每日订单截止时间为16点整,部分城市可到17点整,因超过截止时间造成当日不能发货的将于次日安排发货。
500万彩票5、请办理完货款后,将收据、底单等连同收货人的地址、姓名、等以传真、、短信、等形式通知我们。

产品相关关键字: 四季青 无噬菌体 低内毒素特级胎牛血清 胎牛血清 细胞培养 培养基
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北京索莱宝科技有限公司 专业提供:(HRP_FITC_RBITC_生物素)标记羊抗人二抗 ,(DNA_RNA_质粒_酶)检测试剂盒,彩虹光谱蛋白maker,吉姆萨染色液,Rutin芦丁标准品,西班牙琼脂糖 等产品
北京索莱宝科技有限公司 500万彩票 总访问量:432451 地址:北京通州区马驹桥联东U谷景盛南四街15号85A三层 邮编:100000
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